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The cell death-inducing activity of RmCP. (A) Nicotiana tabacum leaf and (B) Hevea brasiliensis leaf were infiltrated with RmCP and control buffer (0.05 M sodium phosphate, 0.15 M NaCl, pH 7.2; CB) and photographed on the fifth day post infiltration (dpi). Leaf area infiltrated with RmCP displayed clear necrotic lesion while the area infiltrated with CB appeared clear of any lesion. (C) Microscopic observation of cell death in N. tabacum and H. brasiliensis leaves infiltrated with CB and RmCP. Leaves were <t>stained</t> with <t>trypan</t> <t>blue</t> at 24 h post infiltration (hpi) and observed under light microscope. Dead leaf cells were stained blue.
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Thermo Fisher 0.4% trypan blue staining solution
The cell death-inducing activity of RmCP. (A) Nicotiana tabacum leaf and (B) Hevea brasiliensis leaf were infiltrated with RmCP and control buffer (0.05 M sodium phosphate, 0.15 M NaCl, pH 7.2; CB) and photographed on the fifth day post infiltration (dpi). Leaf area infiltrated with RmCP displayed clear necrotic lesion while the area infiltrated with CB appeared clear of any lesion. (C) Microscopic observation of cell death in N. tabacum and H. brasiliensis leaves infiltrated with CB and RmCP. Leaves were <t>stained</t> with <t>trypan</t> <t>blue</t> at 24 h post infiltration (hpi) and observed under light microscope. Dead leaf cells were stained blue.
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Thermo Fisher trypan blue staining solution
The cell death-inducing activity of RmCP. (A) Nicotiana tabacum leaf and (B) Hevea brasiliensis leaf were infiltrated with RmCP and control buffer (0.05 M sodium phosphate, 0.15 M NaCl, pH 7.2; CB) and photographed on the fifth day post infiltration (dpi). Leaf area infiltrated with RmCP displayed clear necrotic lesion while the area infiltrated with CB appeared clear of any lesion. (C) Microscopic observation of cell death in N. tabacum and H. brasiliensis leaves infiltrated with CB and RmCP. Leaves were <t>stained</t> with <t>trypan</t> <t>blue</t> at 24 h post infiltration (hpi) and observed under light microscope. Dead leaf cells were stained blue.
Trypan Blue Staining Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/trypan blue staining solution/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
trypan blue staining solution - by Bioz Stars, 2026-03
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Beijing Solarbio Science trypan blue stain solution
The cell death-inducing activity of RmCP. (A) Nicotiana tabacum leaf and (B) Hevea brasiliensis leaf were infiltrated with RmCP and control buffer (0.05 M sodium phosphate, 0.15 M NaCl, pH 7.2; CB) and photographed on the fifth day post infiltration (dpi). Leaf area infiltrated with RmCP displayed clear necrotic lesion while the area infiltrated with CB appeared clear of any lesion. (C) Microscopic observation of cell death in N. tabacum and H. brasiliensis leaves infiltrated with CB and RmCP. Leaves were <t>stained</t> with <t>trypan</t> <t>blue</t> at 24 h post infiltration (hpi) and observed under light microscope. Dead leaf cells were stained blue.
Trypan Blue Stain Solution, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Procell Inc trypan blue staining solution
The cell death-inducing activity of RmCP. (A) Nicotiana tabacum leaf and (B) Hevea brasiliensis leaf were infiltrated with RmCP and control buffer (0.05 M sodium phosphate, 0.15 M NaCl, pH 7.2; CB) and photographed on the fifth day post infiltration (dpi). Leaf area infiltrated with RmCP displayed clear necrotic lesion while the area infiltrated with CB appeared clear of any lesion. (C) Microscopic observation of cell death in N. tabacum and H. brasiliensis leaves infiltrated with CB and RmCP. Leaves were <t>stained</t> with <t>trypan</t> <t>blue</t> at 24 h post infiltration (hpi) and observed under light microscope. Dead leaf cells were stained blue.
Trypan Blue Staining Solution, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Keygen Biotech trypan blue staining solution kgl2320-12
The cell death-inducing activity of RmCP. (A) Nicotiana tabacum leaf and (B) Hevea brasiliensis leaf were infiltrated with RmCP and control buffer (0.05 M sodium phosphate, 0.15 M NaCl, pH 7.2; CB) and photographed on the fifth day post infiltration (dpi). Leaf area infiltrated with RmCP displayed clear necrotic lesion while the area infiltrated with CB appeared clear of any lesion. (C) Microscopic observation of cell death in N. tabacum and H. brasiliensis leaves infiltrated with CB and RmCP. Leaves were <t>stained</t> with <t>trypan</t> <t>blue</t> at 24 h post infiltration (hpi) and observed under light microscope. Dead leaf cells were stained blue.
Trypan Blue Staining Solution Kgl2320 12, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The cell death-inducing activity of RmCP. (A) Nicotiana tabacum leaf and (B) Hevea brasiliensis leaf were infiltrated with RmCP and control buffer (0.05 M sodium phosphate, 0.15 M NaCl, pH 7.2; CB) and photographed on the fifth day post infiltration (dpi). Leaf area infiltrated with RmCP displayed clear necrotic lesion while the area infiltrated with CB appeared clear of any lesion. (C) Microscopic observation of cell death in N. tabacum and H. brasiliensis leaves infiltrated with CB and RmCP. Leaves were stained with trypan blue at 24 h post infiltration (hpi) and observed under light microscope. Dead leaf cells were stained blue.

Journal: Frontiers in Microbiology

Article Title: RmCP, a cerato-platanin protein from Rigidoporus microporus , induces defense responses during interaction with Hevea brasiliensis

doi: 10.3389/fmicb.2025.1553350

Figure Lengend Snippet: The cell death-inducing activity of RmCP. (A) Nicotiana tabacum leaf and (B) Hevea brasiliensis leaf were infiltrated with RmCP and control buffer (0.05 M sodium phosphate, 0.15 M NaCl, pH 7.2; CB) and photographed on the fifth day post infiltration (dpi). Leaf area infiltrated with RmCP displayed clear necrotic lesion while the area infiltrated with CB appeared clear of any lesion. (C) Microscopic observation of cell death in N. tabacum and H. brasiliensis leaves infiltrated with CB and RmCP. Leaves were stained with trypan blue at 24 h post infiltration (hpi) and observed under light microscope. Dead leaf cells were stained blue.

Article Snippet: Leaf tissues were immersed in 0.5% trypan blue stain solution (Nacalai Tesque, Japan) for an hour followed by rinsing in absolute ethanol.

Techniques: Activity Assay, Control, Staining, Light Microscopy